 |
7365‑45‑9
|
| |
Packaging & Storage |
Packaging: 25 kg/drum. Custom packaging can be discussed based on order quantity and transportation requirements.
Storage: Store in a cool, dry place, keep container tightly sealed, protect from light and moisture.
Storage & Handling Notes
For powdered HEPES, prolonged exposure to high humidity, moisture or direct sunlight may cause the material to absorb moisture and agglomerate. Keep container tightly sealed at all times.
Shipping & Delivery |
Jinan, China
Immediately after payment
Qingdao / Tianjin / Shanghai / Ningbo
Sample Support
Samples are available for evaluation before bulk orders. Shipment arrangements depend on destination and applicable transportation requirements.
Factory & Quality Control |
Consistent quality control for each production batch.
Technical documents available upon request.
Samples available for product evaluation.
Custom specifications available upon request.
View Factory & Quality Control → View Technical Documents →
Applications |
Applications of HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid)
HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, CAS 7365-45-9) is a zwitterionic buffering agent widely used as a biological buffer building-block in cell-culture media and diagnostic reagent formulations. Its molecular structure makes it particularly suitable for CO₂-independent cell incubation, biochemical assay systems, and selected high-performance biopharmaceutical processes.
Cell Culture & Biological Research
HEPES is commonly used in the production of mammalian cell-culture media. Its zwitterionic structure can support stable pH buffering capacity, low metal-ion chelating effect, and broad formulation compatibility, making it suitable for eukaryotic cell culture applications.
Typical application areas include:
Mammalian cell culture media
CO₂-independent incubation systems
Cell-based biological research
Primary cell and tumor cell cultivation
In vitro Diagnostic Reagents & Biochemical Assays
HEPES can be used in buffer systems designed for in-vitro diagnostic kits, biochemical detection and immunoassay platforms. HEPES-based buffer formulations may provide a useful balance of biocompatibility and assay performance, including pH stability under ambient atmosphere, low cytotoxicity, and good compatibility with enzymes and antigen-antibody reactions.
It is particularly suitable for formulations where reliable pH stability, minimal interference to bio-reactions, and long-term liquid reagent stability are required.
HEPES is also used as a buffer component in protein purification and biopharmaceutical processing. These buffer systems are applied in biomolecule preparation and applications requiring gentle physiological pH conditions.
Typical applications include:
Protein and enzyme purification
Biopharmaceutical buffer preparation
Immunoassay diagnostic kits
Biochemical laboratory reagent
Bio-Reagent Formulation & Downstream Biological Applications
HEPES-based buffer systems can be used in bio-reagent and biological sample preservation applications, particularly where non-toxic buffering environment and consistent physiological pH maintenance are important performance considerations.
The final performance of HEPES in any buffer or reagent system depends on the complete formulation, salt ratio, processing conditions, sterilization method, and intended end use. Customers should evaluate product suitability according to their specific formulation and application requirements.
![2,2'-Azobis([2'(2-Imidazolin-2-Yl)Propane] Dihydrochloride](http://imrorwxhomrrlk5q-static.micyjz.com/cloud/poBpnKomlmSRmlmkkjklkm/2-2-Azobis-2-2-Imidazolin-2-Yl-Propane-Dihydrochloride-640-640.jpg)
